Simplified sugar fermentation plate technique for identification of Neisseria gonorrhoeae.
نویسندگان
چکیده
A simplified sugar fermentation technique incorporating (i) a glucose and (ii) a maltose, lactose, and sucrose plate was developed for confirming large numbers of Neisseria gonorrhoeae cultures.
منابع مشابه
Identification of Neisseria gonorrhoeae in the routine venerelogical laboratory: Comparative study of coagglutination, direct immunofluorescence, and sugar fermentation reaction.
The coagglutination (CoA) method for the identification of Neisseria gonorrhoeae colonies grown on selective culture media was used on 116 strains in a routine venereological laboratory together with the direct immunofluorescence (IF) test and the sugar fermentation reaction. Correlation of results between the CoA method and the direct IF test and between the CoA test and the sugar fermentation...
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A carbohydrate fermentation plate medium is described for rapid and reliable confirmation of Neisseria gonorrhoeae, N. meningitidis, and other Neisseria species. The medium is based on a modification of NYC (U.S. Patent 3,846,241) medium, originally designed for the isolation of pathogenic Neisseria (3). A total of 715 clinical isolates were tested for their carbohydrate fermentation reactions ...
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A modification of the rapid sugar fermentation procedure has been developed because of difficulties encountered in reproducing the reported results. Changes in the method have made the test more sensitive and reproducible.
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A rapid sugar fermentation procedure has been developed for the confirmation of Neisseria gonorrhoeae from either primary isolation media or purification media. A lightly buffered salt solution with pH indicator and sugars was heavily inoculated with presumptively positive growth. Proper fermentation patterns are obtained in 1 to 4 h with no interference from inhibited contaminants or variation...
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The carbohydrate fermentation test in cystine trypticase agar (BBL)-tubed medium was compared with a simple method using commercially available carbohydrate impregnated disks on culture inoculated Thayer-Martin medium with and without (vancomycin, colistimethate, and nystatin) inhibitors. There was 100% agreement between the two methods when a limited sample of clinical isolates of Neisseria go...
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ورودعنوان ژورنال:
- Applied microbiology
دوره 27 2 شماره
صفحات -
تاریخ انتشار 1974